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KMID : 1234420080360030227
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 3 p.227 ~ p.232
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Antifungal Activity of Bacillus sp. AM-651 Against Phytophthora capsici
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Lee Jung-Bok
Shin Jeong-Hak
Jang Jong-Ok
Shin Kee-Sun
Choi Chung-Sig
Kim Kun-Woo
Cho Min-Seop
Jeon Chun-Pyo
Kim Yun-Hoi
Kwon Gi-Seok
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Abstract
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Biological antagonists of Phytophthora capsici were isolated from soil in Gyeongbuk, Korea. Among the isolated bacteria, a Bacillus sp. was identified from 16S rDNA sequence analysis and named Bacillus sp. AM-651. Bacillus sp. AM-651 strain which can strongly a antifungal activity against Phytophthora capsici. Culture conditions for the maximum production of the antagonistic substance were optimized. The production of antibiotic were high on modified Davis mineral medium pH 7 at 30oC. The medium for highest production of the agonistic substance optimized. It is composed the best activity on glucose, (NH4)2SO4 and K2HPO4 at 0.5%, 0.1%, and 0.7%, respectively. By time course of culture solution selected Bacillus sp. AM-651, the culture solution after 48hrs had strongly growth inhibition rate against P. capsici. And culture solution of Bacillus sp. AM-651 was stable within a pH range 5~11 and temperature range 4~70oC. Bacillus sp. AM-651 cultured broth shown fungal growth inhibitory activitiy against B. sorokiniana, B. cinerea, R. solani avove and beyond P. capsici and comparatively showed a high activity against C. gloeosporioides, B. dothidea, B. cinerea and F. graminearum by agar diffusion method.
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KEYWORD
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Antifungal, antibiotic, Bacillus sp, AM-651, Phytiphthora capsici, biopestcide
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